Comparison of the Simple Cyclic Voltammetry (CV) and DPPH Assays for the Determination of Antioxidant Capacity of Active Principles

Molecules

Jesús F. Arteaga¹  Mercedes Ruiz-Montoya¹  Alberto Palma¹  Gema Alonso-Garrido¹  Sara Pintado¹ 

[1] ¹Department of Chemical Engineering, Physical Chemistry and Organic Chemistry, University of Huelva, Campus de El Carmen, 21071 Huelva, Spain ²Department of Physical Chemistry and Applied Thermodynamic, Córdoba University, Campus de Excelencia Internacional Agroalimentario (CEIA3), ed. Marie Curie, 14014 Córdoba, Spain

关键词: antioxidant activity;    cyclic voltammetry;    DPPH;    phenolics;    low weight antioxidants;   

DOI  :  10.3390/molecules17055126

来源: mdpi

【 摘 要 】

Antioxidant activity of a number of small (low molecular weight) natural compounds found in spices, condiments or drugs (gallic acid, sesamol, eugenol, thymol, carvacrol, vanillin, salicylaldehyde, limonene, geraniol, 4-hexylresorcinol, etc.) has been evaluated using electrochemical and DPPH^• radical scavenging measurements. Structural analysis of the tested compound suggest a remarkable activity for phenol derivatives and the importance of the –R groups located on the phenolic ring in the molecule’s ability to act as free radical scavenging as well as their influence in the electrochemical behavior. The voltammetric method can be used for the determination of the antioxidant capability in the same manner as the DPPH^• radical scavenging because of the correlation found between oxidation potentials and anti-radical power (ARP = 1/EC₅₀). Such electrochemical determination is fast and cheap and allows making measurements under a variety of experimental conditions. The accuracy of the electrochemical measurements is the same for all the compounds, irrespective of their scavenging activity, the opposite of what occurs in the DPPH^• test.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.