Lipid Oxidation Inhibitory Effects and Phenolic Composition of Aqueous Extracts from Medicinal Plants of Colombian Amazonia

International Journal of Molecular Sciences

Leandro J. Lizcano²  Mar໚ Viloria-Bernal³  Francisca Vicente³  Luis Angel Berrueta³  Blanca Gallo³  Magdalena Martínez-Ca༚mero¹  Maria Bego༚ Ruiz-Larrea² 

[1] Department of Health Sciences, Microbiology Area, Experimental Sciences School, University of Jaen, 23071-Jaen, Spain; E-Mail:;Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain; E-Mails:;Department of Analytical Chemistry, Sciences and Technology School, University of the Basque Country UPV/EHU, 48940-Leioa, Spain; E-Mails:

关键词: polyphenols;    lipid peroxidation;    liver microsomes;    HPLC-DAD-MS/MS;    Amazonian plants;   

DOI  :  10.3390/ijms13055454

来源: mdpi

【 摘 要 】

Diverse plants of ethnobotanic interest in Amazonia are commonly used in traditional medicine. We determined the antioxidant potential against lipid peroxidation, the antimicrobial activity, and the polyphenol composition of several Amazonian plants (Brownea rosademonte, Piper glandulosissimum, Piper krukoffii, Piper putumayoense, Solanum grandiflorum, and Vismia baccifera). Extracts from the plant leaf, bark, and stem were prepared as aqueous infusions, as used in folk medicine, and added to rat liver microsomes exposed to iron. The polyphenolic composition was detected by reverse-phase HPLC coupled to diode-array detector and MS/MS analysis. The antimicrobial activity was tested by the spot-on-a-lawn method against several indicator microorganisms. All the extracts inhibited lipid oxidation, except the P. glandulosissimum stem. The plant extracts exhibiting high antioxidant potential (V. baccifera and B. rosademonte) contained high levels of flavanols (particularly, catechin and epicatechin). By contrast, S. grandiflorum leaf, which exhibited very low antioxidant activity, was rich in hydroxycinnamic acids. None of the extracts showed antimicrobial activity. This study demonstrates for the first time the presence of bioactive polyphenolic compounds in several Amazonian plants, and highlights the importance of flavanols as major phenolic contributors to antioxidant activity.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.