Immunostimulatory Effect of Laminarin on RAW 264.7 Mouse Macrophages

Molecules

Ji Young Lee¹  Young-Jin Kim¹  Hyun Joo Kim¹  Yoon-Sang Kim¹ 

[1] College of Korean Medicine, Gachon University, Seongnam 461-701, Korea

关键词: laminarin;    macrophage;    immunostimulatory;    cytokine;    nitric oxide;    hydrogen peroxide;    calcium;    transcription factor;   

DOI  :  10.3390/molecules17055404

来源: mdpi

【 摘 要 】

This study investigated the immunostimulatory effects of laminarin with respect to inflammatory mediators such as hydrogen peroxide, calcium, nitric oxide, various cytokines, transcription factors, and immune response gene in RAW 264.7 mouse macrophages. Laminarin did not reduce the cell proliferation of RAW 264.7 mouse macrophages at concentrations up to 500 µg/mL. Laminarin significantly increased the release of hydrogen peroxide, calcium, nitric oxide, monocyte chemotactic protein-1, vascular endothelial growth factor, leukemia inhibitory factor, and granulocyte-colony stimulating factor with enhancing expression of Signal Transducer and Activator of Transcription 1 (STAT1), STAT3, c-Jun, c-Fos, and cyclooxygenase-2 mRNA in RAW 264.7 cells. The results suggest that laminarin has immunostimulatory properties, strengthening immune reactions via the transcription factor pathway in macrophages.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.