Immunomodulatory Activity of Recombinant Ricin Toxin Binding Subunit B (RTB)

International Journal of Molecular Sciences

Wensen Liu¹  Na Xu¹  Hongyan Yuan²  Songyan Li¹  Linna Liu¹  Zhaoyang Pu¹  Jiayu Wan¹  Huiwen Wang²  Yaping Chang² 

[1] Institute of Military Veterinary, Academy of Military Medical Sciences, Zoonosis Prevention and Control Key Laboratory, Changchun 130122, China; E-Mails:;Department of Immunology, Norman Bethune College of Medical Science, Jilin University, Changchun 130021, China; E-Mails:

关键词: RTB;    macrophage;    lymphocyte proliferation;    Th1 cell;    cytokine;   

DOI  :  10.3390/ijms140612401

来源: mdpi

【 摘 要 】

Ricin toxin binding subunit B (RTB) is one of the subunits of the ricin protein. RTB has been used as adjuvant, but little is known about its mechanism. In this study, we found that RTB increased not only nitric oxide (NO) release, but also tumor necrosis factor (TNF)-α and interleukin (IL)-6 production in mouse macrophage cell line RAW264.7 cells. They subsequently exhibited enhanced ConA-induced T-cell and LPS-induced B-cell proliferative responses. We also examined the cytokines that were produced from splenocytes following in vitro RTB administration. Increased levels of IL-2, interferon (IFN)-γ and TNF-α were observed, while IL-4 and IL-5 were unaffected. These results demonstrate that recombinant RTB can act on the immune system and activate T-cells by introducing a Th1 immune response. Th1 cells might be the primary cellular target affected by RTB. Our results suggest that the recombinant RTB can promote the activation of macrophages and has a beneficial effect on immunomodulatory activity.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland