A Fast Response Highly Selective Probe for the Detection of Glutathione in Human Blood Plasma

Sensors

Yixing Guo²  Xiaofeng Yang¹  Lovemore Hakuna²  Aabha Barve²  Jorge O. Escobedo²  Mark Lowry² 

[1] Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Analytical Sciences, College of Chemistry & Materials Science, Northwest University, Xi'an 710069, China; E-Mail:;Department of Chemistry, Portland State University, Portland, OR 97201, USA; E-Mails:

关键词: fluorescent probe;    glutathione;    micelle-catalyzed;    selectivity;   

DOI  :  10.3390/s120505940

来源: mdpi

【 摘 要 】

A fluorescent probe for glutathione (GSH) detection was developed. Our study indicates a possible mechanism which couples a conjugate addition and micelle-catalyzed large membered ring formation/elimination sequence. This method enables excellent selectivity towards GSH over other biological thiols such as cysteine (Cys) and homocysteine (Hcy). The proposed method is precise with a relative standard deviation (R.S.D) lower than 6% (n = 3) and has been successfully applied to determine GSH in human plasma with recoveries between 99.2% and 102.3%.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.