Amino Acid and Peptide Immobilization on Oxidized Nanocellulose: Spectroscopic Characterization

Nanomaterials

Sa໽ Barazzouk¹ 

关键词: oxidized nanocellulose;    amino acids;    peptides;    amide bond;    spectroscopic characterization;   

DOI  :  10.3390/nano2020187

来源: mdpi

【 摘 要 】

In this work, oxidized nanocellulose (ONC) was synthesized and chemically coupled with amino acids and peptides using a two step coupling method at room temperature. First, ONC was activated by N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride, forming a stable active ester in the presence of N-hydroxysuccinimide. Second, the active ester was reacted with the amino group of the amino acid or peptide, forming an amide bond between ONC and the grafted molecule. Using this method, the intermolecular interaction of amino acids and peptides was avoided and uniform coupling of these molecules on ONC was achieved. The coupling reaction was very fast in mild conditions and without alteration of the polysaccharide. The coupling products (ONC-amino acids and ONC-peptides) were characterized by transmission electron microscopy and by the absorption, emission, Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) spectroscopic techniques.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.