Effects of Purified Saccharomyces cerevisiae (1→3)-β-Glucan on Venous Ulcer Healing

International Journal of Molecular Sciences

Sarah Dantas Viana Medeiros¹  Sara Lima Cordeiro³  Jéssica Escorel Chaves Cavalcanti¹  Karina Mendes Melchuna¹  Aleida Maria da Silva Lima¹  Irami Araújo Filho⁶  Aldo Cunha Medeiros⁶  Keyla Borges Ferreira Rocha⁴  Elizabeth Maia Oliveira⁴  Eduardo Dantas Baptista Faria⁵  Guilherme Lanzi Sassaki²  Hugo Alexandre Oliveira Rocha³ 

[1] Laboratory of Clinical Immunology, Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil; E-Mails:;Department of Biochemistry and Molecular Biology, Federal University of Paraná (UFPR), Cento Politécnico S/N, Jardim das Américas, Curitiba, PR 81531-990, Brazil; E-Mail:;Laboratory of Biotechnology of Natural Polymers (BIOPOL), Department of Biochemistry, Federal University of Rio Grande do Norte (UFRN), 3000, Salgado Filho Ave., Natal, RN 59078-970, Brazil; E-Mails:;Laboratory of Pathology, Department of Pathology, Federal University of Rio Grande do Norte (UFRN), General Gustavo Cordeiro de Farias Ave., Petrópolis, Natal, RN 59012-570, Brazil; E-Mails:;University Hospital Onofre Lopes, Department of Integrated Medicine, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil; E-Mail:;University Hospital Onofre Lopes, Department of Surgery, Federal University of Rio Grande do Norte (UFRN), 620, Nilo Peçanha st., Petrópolis, Natal, RN 59012-300, Brazil; E-Mails:

关键词: water-insoluble glucan;    polysaccharide;    yeast;    immunomodulator;    tissue repair;   

DOI  :  10.3390/ijms13078142

来源: mdpi

【 摘 要 】

Water-insoluble glucan was isolated from the baker’s yeast Saccharomyces cerevisiae. The yeast cells were treated with alkali and the residue then with acid. Chemical and NMR (1D and 2D) analyses showed that a linear (1→3)-β-glucan was purified that was not contaminated with other carbohydrates, proteins or phenolic compounds. The effects of the glucan on wound healing were assessed in human venous ulcers by histopathological analysis after 30 days of topical treatment. (1→3)-β-glucan enhanced ulcer healing and increased epithelial hyperplasia, as well as increased inflammatory cells, angiogenesis and fibroblast proliferation. In one patient who had an ulcer that would not heal for over 15 years, glucan treatment caused a 67.8% decrease in the area of the ulcer. This is the first study to investigate the effects of (1→3)-β-glucan on venous ulcer healing in humans; our findings suggest that this glucan is a potential natural biological response modifier in wound healing.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.