Genome-Wide Sequencing Reveals Two Major Sub-Lineages in the Genetically Monomorphic Pathogen Xanthomonas Campestris Pathovar Musacearum

Genes

Arthur Wasukira²  Johnbosco Tayebwa²  Richard Thwaites³  Konrad Paszkiewicz²  Valente Aritua¹  Jerome Kubiriba¹  Julian Smith³  Murray Grant² 

[1] National Crops Resources Research Institute (NaCRRI), Kampala 7084, Uganda; E-Mails:;Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK; E-Mails:;The Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK; E-Mails:

关键词: banana Xanthomonas wilt;    whole-genome sequencing;    SNP;    molecular markers;    Ensete ventricosum;    Xanthomonas vasicola;   

DOI  :  10.3390/genes3030361

来源: mdpi

【 摘 要 】

The bacterium Xanthomonas campestris pathovar musacearum (Xcm) is the causal agent of banana Xanthomonas wilt (BXW). This disease has devastated economies based on banana and plantain crops (Musa species) in East Africa. Here we use genome-wide sequencing to discover a set of single-nucleotide polymorphisms (SNPs) among East African isolates of Xcm. These SNPs have potential as molecular markers for phylogeographic studies of the epidemiology and spread of the pathogen. Our analysis reveals two major sub-lineages of the pathogen, suggesting that the current outbreaks of BXW on Musa species in the region may have more than one introductory event, perhaps from Ethiopia. Also, based on comparisons of genome-wide sequence data from multiple isolates of Xcm and multiple strains of X. vasicola pathovar vasculorum, we identify genes specific to Xcm that could be used to specifically detect Xcm by PCR-based methods.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.