Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum

Biosensors

Mathieu Lazerges¹  Hubert Perrot¹  Niriniony Rabehagasoa¹ 

[1] Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France; E-Mails:

关键词: Alexandrium Minutum;    avidin;    biosensor;    biotin;    DNA;    quartz crystal miocrobalance;    self-assembled monolayer;    thiol;   

DOI  :  10.3390/bios2030245

来源: mdpi

【 摘 要 】

Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.

【 授权许可】

CC BY   
© 2012 by MDPI, Basel, Switzerland