Pre-Ischemic Treadmill Training for Prevention of Ischemic Brain Injury via Regulation of Glutamate and Its Transporter GLT-1

International Journal of Molecular Sciences

Xiaojiao Yang³  Zhijie He³  Qi Zhang³  Yi Wu³  Yongshan Hu³  Xiaolou Wang¹  Mingfen Li³  Zhiyuan Wu³  Zhenzhen Guo³  Jingchun Guo² 

[1] The Third Teaching Hospital of Xinxiang Medical University, Xinxiang 453003, China; E-Mail:;State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China;Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China; E-Mails:

关键词: glutamate;    glutamate transporter-1 (GLT-1);    pre-ischemic treadmill training;    ischemia;    neuroprotection;   

DOI  :  10.3390/ijms13089447

来源: mdpi

【 摘 要 】

Pre-ischemic treadmill training exerts cerebral protection in the prevention of cerebral ischemia by alleviating neurotoxicity induced by excessive glutamate release following ischemic stroke. However, the underlying mechanism of this process remains unclear. Cerebral ischemia-reperfusion injury was observed in a rat model after 2 weeks of pre-ischemic treadmill training. Cerebrospinal fluid was collected using the microdialysis sampling method, and the concentration of glutamate was determined every 40 min from the beginning of ischemia to 4 h after reperfusion with high-performance liquid chromatography (HPLC)-fluorescence detection. At 3, 12, 24, and 48 h after ischemia, the expression of the glutamate transporter-1 (GLT-1) protein in brain tissues was determined by Western blot respectively. The effect of pre-ischemic treadmill training on glutamate concentration and GLT-1 expression after cerebral ischemia in rats along with changes in neurobehavioral score and cerebral infarct volume after 24 h ischemia yields critical information necessary to understand the protection mechanism exhibited by pre-ischemic treadmill training. The results demonstrated that pre-ischemic treadmill training up-regulates GLT-1 expression, decreases extracellular glutamate concentration, reduces cerebral infarct volume, and improves neurobehavioral score. Pre-ischemic treadmill training is likely to induce neuroprotection after cerebral ischemia by regulating GLT-1 expression, which results in re-uptake of excessive glutamate.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.