Molecules | |
Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery | |
Pai Feng Tsai3  Wei Yang Chang2  Yu Che Hsiao1  Kuo Jung Li2  | |
[1] Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN 37996, USA;Department of Biotechnology, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec. 1, Jen-Te, Taiwan;Department of Occupational Safety and Hygiene, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Rd., Sec. 1, Jen-Te, Taiwan; | |
关键词: poly(aminoester); folic acid; transfection; cytotoxicity; | |
DOI : 10.3390/molecules17089056 | |
来源: mdpi | |
【 摘 要 】
A new poly(aminoester) (EPAE-FA) containing folic acid and amino groups in the backbone and side chain was synthesized. EPAE-FA self-assembled readily with the plasmid DNA (pCMV-βgal) in HEPES buffer and was characterized by dynamic light scattering, zeta potential, fluorescence images, and XTT cell viability assays. To evaluate the transfection effect of graft ratio of FA on the EPAE system, EPAE-FA polymers with two different graft ratios (EPAE-FA12k and EPAE-FA14k) were also prepared. This study found that all EPAE-FA polymers were able to bind plasmid DNA and yielded positively charged complexes with nano-sized particles (<200 nm). To assess the transfection efficiency mediated by EPAE and EPAE-FA polymers, we performed
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190042842ZK.pdf | 608KB | download |