In Vitro Anti-diabetic Activities and Chemical Analysis of Polypeptide-k and Oil Isolated from Seeds of Momordica charantia (Bitter Gourd)

Molecules

Zuraini Ahmad¹  Khairul Faizi Zamhuri¹  Azhar Yaacob³  Chiong Hoe Siong¹  Malarvili Selvarajah¹  Amin Ismail² 

[1] Department of Biomedical Sciences, Universiti Putra Malaysia, 43400 UPM Serdang Selangor, Malaysia;Department of Nutrition and Dietetics, Universiti Putra Malaysia, 43400 UPM Serdang Selangor, Malaysia;Sports Academy, Universiti Putra Malaysia, 43400 UPM Serdang Selangor, Malaysia;

关键词: Momordica charantia;    amino acids;    fatty acids;    α-glucosidase;    α-amylase;   

DOI  :  10.3390/molecules17089631

来源: mdpi

【 摘 要 】

The amino acid and fatty acid composition of polypeptide k and oil isolated from the seeds of Momordica charantia was analysed. The analysis revealed polypeptide k contained 9 out of 11 essential amino acids, among a total of 18 types of amino acids. Glutamic acid, aspartic acid, arginine and glycine were the most abundant (17.08%, 9.71%, 9.50% and 8.90% of total amino acids, respectively). Fatty acid analysis showed unusually high amounts of C18-0 (stearic acid, 62.31% of total fatty acid). C18-1 (oleic acid) and C18-2 (linoleic acid) were the other major fatty acid detected (12.53% and 10.40%, respectively). The oil was devoid of the short fatty acids (C4-0 to C8-0). Polypeptide k and oil were also subjected to in vitro α-glucosidase and α-amylase inhibition assays. Both polypeptide k and seed oil showed potent inhibition of α-glucosidase enzyme (79.18% and 53.55% inhibition, respectively). α-Amylase was inhibited by 35.58% and 38.02%, respectively. Collectively, the in vitro assay strongly suggests that both polypeptide k and seed oil from Momordica charantia are potent potential hypoglycemic agents.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.