Development of a Plastic-Based Microfluidic Immunosensor Chip for Detection of H1N1 Influenza

Sensors

Kyoung G. Lee¹  Tae Jae Lee¹  Soon Woo Jeong¹  Ho Woon Choi¹  Nam Su Heo⁴  Jung Youn Park²  Tae Jung Park³ 

[1] Center for Nanobio Integration & Convergence Engineering (NICE), National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon 305-806, Korea; E-Mails:;Biotechnology Research Division, National Fisheries Research & Development Institute (NFRDI), 408-1 Sirang-ri, Gijang, Busan 619-705, Korea; E-Mail:;Department of Chemistry, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 156-756, Korea;Bioprocess Engineering Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Korea; E-Mail:

关键词: immunosensor;    H1N1 influenza;    microfluidics;    plastic chip;   

DOI  :  10.3390/s120810810

来源: mdpi

【 摘 要 】

Lab-on-a-chip can provide convenient and accurate diagnosis tools. In this paper, a plastic-based microfluidic immunosensor chip for the diagnosis of swine flu (H1N1) was developed by immobilizing hemagglutinin antigen on a gold surface using a genetically engineered polypeptide. A fluorescent dye-labeled antibody (Ab) was used for quantifying the concentration of Ab in the immunosensor chip using a fluorescent technique. For increasing the detection efficiency and reducing the errors, three chambers and three microchannels were designed in one microfluidic chip. This protocol could be applied to the diagnosis of other infectious diseases in a microfluidic device.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.