Application of HPLC-NMR in the Identification of Plocamenone and Isoplocamenone from the Marine Red Alga Plocamium angustum

Marine Drugs

Michael Anthony Timmers²  Daniel Anthony Dias¹ 

[1] School of Applied Sciences, Health Innovations Research Institute (HIRi), RMIT University, GPO Box 2476V, Melbourne, Victoria 3001, Australia;

关键词: Plocamium angustum;    polyhalogenated acyclic monoterpenes;    hyphenated spectroscopy;    HPLC-NMR;    biological activity;   

DOI  :  10.3390/md10092089

来源: mdpi

【 摘 要 】

A combination of on-line HPLC-NMR and off-line chemical investigations has resulted in the identification of the previously reported polyhalogenated monoterpene plocamenone, together with the new structural analogue isoplocamenone from the crude extract of the marine alga Plocamium angustum. On-flow and stop-flow HPLC-NMR analyses (including the acquisition of WET 2D NMR spectra) rapidly assisted in the identification of the major component plocamenone and in the partial identification of its unstable double bond isomer isoplocamenone. Conventional off-line isolation and structural characterization techniques were employed to unequivocally confirm both structures, leading to a structural revision for plocamenone, as well as to obtain sufficient quantities for biological testing.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.