Identification of Three New N-Demethylated and O-Demethyled Bisbenzylisoquinoline Alkaloid Metabolites of Isoliensinine from Dog Hepatic Microsomes

Molecules

Hui Zhou¹  Liping Li¹  Huidi Jiang¹ 

[1] id="af1-molecules-17-11712">Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, Zhejiang, Chi

关键词: bisbenzylisoquinoline alkaloids;    demethylation;    HPLC;    isoliensinine;    microsomes;    tandem mass spectrometry;   

DOI  :  10.3390/molecules171011712

来源: mdpi

【 摘 要 】

Isoliensinine, a natural phenolic bisbenzyltetrahydroisoquinoline alkaloid, has received considerable attention for its potential biological effects such as antioxidant and anti-HIV activities. From the dog hepatic microsomes of isoliensinine, three new N-demethylated and O-demethylated metabolites, 2-N-desmethyl-isoliensinine (M1), 2'-N-desmethylisoliensinine (M2), and 2'-N-6-O-didesmethylisoliensinine (M3), were identified by high-performance liquid chromatography and data-dependent electrospray ionization tandem mass spectrometry. Possible metabolic pathways for isoliensinine have been proposed. The result should prove very helpful for evaluation of the drug-like properties of isoliensinine and other bisbenzylisoquinoline alkaloids.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.