Deposition of Bacteriorhodopsin Protein in a Purple Membrane Form on Nitrocellulose Membranes for Enhanced Photoelectric Response

Sensors

Young Jun Kim¹  Pavel Neuzil¹  Chang-Hoon Nam¹ 

[1] Laboratory of Nanomedicine, Korea Institute of Science and Technology Europe (KIST-Europe), Campus E 7.1, Saarbrücken, Germany; E-Mails:

关键词: bacteriorhodopsin (bR);    purple membrane (PM);    indium tin oxide (ITO);    positively charged nitrocellulose membrane (PNM);   

DOI  :  10.3390/s130100455

来源: mdpi

【 摘 要 】

Bacteriorhodopsin protein (bR)-based systems are one of the simplest known biological energy converters. The robust chemical, thermal and electrochemical properties of bR have made it an attractive material for photoelectric devices. This study demonstrates the photoelectric response of a dry bR layer deposited on a nitrocellulose membrane with indium tin oxide (ITO) electrodes. Light-induced electrical current as well as potential and impedance changes of dried bR film were recorded as the function of illumination. We have also tested bR in solution and found that the electrical properties are strongly dependent on light intensity changing locally proton concentration and thus pH of the solution. Experimental data support the assumption that bR protein on a positively charged nitrocellulose membrane (PNM) can be used as highly sensitive photo- and pH detector. Here the bR layer facilitates proton translocation and acts as an ultrafast optoelectric signal transducer. It is therefore useful in applications related to bioelectronics, biosensors, bio-optics devices and current carrying junction devices.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.