Application of Paper-Supported Printed Gold Electrodes for Impedimetric Immunosensor Development

Biosensors

Petri Ihalainen²  Himadri Majumdar³  Tapani Viitala⁴  Björn Törngren²  Tuomas Närjeoja¹  Anni Määttänen²  Jawad Sarfraz²  Harri Härmä¹  Marjo Yliperttula⁴  Ronald Österbacka³ 

[1] Laboratory of Biophysics, Institute of Biomedicine and Medicity Research Laboratories, University of Turku, Turku, Finland; E-Mails:;Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails:;Center of Excellence for Functional Materials and Physics, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mail:;Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland; E-Mails:

关键词: paper electronics;    nanoparticles;    inkjet printing;    immunoassays;    impedance spectroscopy;   

DOI  :  10.3390/bios3010001

来源: mdpi

【 摘 要 】

In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where—(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth—all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.