Optimization of Synthesis, Characterization and Cytotoxic Activity of Seleno-Capparis spionosa L. Polysaccharide

International Journal of Molecular Sciences

Yu-Bin Ji¹  Fang Dong¹  Lang Lang¹  Ling-Wen Zhang¹  Jing Miao²  Zhen-Feng Liu²  Li-Na Jin² 

[1] Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China; E-Mails:;Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin 150076, China; E-Mails:

关键词: seleno-Capparis spionosa L. polysaccharide(Se-CSPS);    reaction optimization;    response surface methodology;    characterization;    cytotoxic activity;   

DOI  :  10.3390/ijms131217275

来源: mdpi

【 摘 要 】

In this study, an experiment was designed to optimize the synthesis of seleno-Capparis spionosa L. polysaccharide (Se-CSPS) by response surface methodology. Three independent variables (reaction time, reaction temperature and ratio of Na₂SeO₃ to CSPS) were tested. Furthermore, the thermal stability, particle size, shape and cytotoxic activity of Se-CSPS in vitro were investigated. The optimum reaction conditions were obtained shown as follows: reaction time 7.5 h, reaction temperature 71 °C, and ratio of Na₂SeO₃ to CSPS 0.9 g/g. Under these conditions, the Se content in Se-CSPS reached 5.547 mg/g, which was close to the predicted value (5.518 mg/g) by the model. The thermal stability, particle size and shape of Se-CSPS were significantly different from those of CSPS. Additionally, a MTT assay indicated that the Se-CSPS could inhibit the proliferation of human gastric cancer SGC-7901 cells in a dose-dependent manner.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.