Comparative ANNs with Different Input Layers and GA-PLS Study for Simultaneous Spectrofluorimetric Determinationof Melatonin and Pyridoxine HCl in the Presence of Melatonin’s Main Impurity

Molecules

Hany W. Darwish¹  Mohamed I. Attia¹  Ali S. Abdelhameed¹  Amer M. Alanazi¹ 

[1]¹Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia

关键词: melatonin;    pyridoxine HCl;    spectrofluorimetry;    multivariate calibration methods;    pharmaceutical tablets;   

DOI  :  10.3390/molecules18010974

来源: mdpi

【 摘 要 】

Melatonin (MLT) has many health implications, therefore it is important to develop specific analytical methods for the determination of MLT in the presence of its main impurity, N-{2-[1-({3-[2-(acetylamino)ethyl]-5-methoxy-1H-indol-2-yl}methyl)-5-methoxy-1H-indol-3-yl]ethyl}acetaamide (DMLT) and pyridoxine HCl (PNH) as aco-formulated drug. This work describes simple, sensitive, and reliable four multivariate calibration methods, namely artificial neural network preceded by genetic algorithm(GA-ANN), principal component analysis (PCA-ANN) and wavelet transform procedures (WT-ANN) as well as partial least squares preceded by genetic algorithm(GA-PLS) for the spectrofluorimetric determination of MLT and PNH in the presence of DMLT. Analytical performance of the proposed methods was statistically validated with respect to linearity, accuracy, precision and specificity. The proposed methods were successfully applied for the assay of MLT in laboratory prepared mixtures containing up to 15% of DMLT and in commercial MLT tablets with recoveries of no less than 99.00%. Nointerference was observed from common pharmaceutical additives and the results compared favorably with those obtained by a reference method.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.