Comparative Proteomic Analysis of Puccinellia tenuiflora Leaves under Na2CO3 Stress

International Journal of Molecular Sciences

Juanjuan Yu²  Sixue Chen⁴  Tai Wang¹  Guorong Sun³ 

[1] Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; E-Mail:;College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China; E-Mail:;Binzhou Polytechnic College, Binzhou 256603, Shandong, China; E-Mail:;Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, Gainesville, FL 32610, USA; E-Mail:

关键词: proteomics;    halophyte;    Puccinellia tenuiflora;    Na₂CO₃ response;   

DOI  :  10.3390/ijms14011740

来源: mdpi

【 摘 要 】

Soil salt-alkalinization is a widespread environmental stress that limits crop growth and agricultural productivity. The influence of soil alkalization caused by Na₂CO₃ on plants is more severe than that of soil salinization. Plants have evolved some unique mechanisms to cope with alkali stress; however, the plant alkaline-responsive signaling and molecular pathways are still unknown. In the present study, Na₂CO₃ responsive characteristics in leaves from 50-day-old seedlings of halophyte Puccinellia tenuiflora were investigated using physiological and proteomic approaches. Comparative proteomics revealed 43 differentially expressed proteins in P. tenuiflora leaves in response to Na₂CO₃ treatment for seven days. These proteins were mainly involved in photosynthesis, stress and defense, carbohydrate/energy metabolism, protein metabolism, signaling, membrane and transport. By integrating the changes of photosynthesis, ion contents, and stress-related enzyme activities, some unique Na₂CO₃ responsive mechanisms have been discovered in P. tenuiflora. This study provides new molecular information toward improving the alkali tolerance of cereals.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.