Discovery and Characterization of Two Novel Salt-Tolerance Genes in Puccinellia tenuiflora

International Journal of Molecular Sciences

Ying Li¹  Tetsuo Takano² 

[1] Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin Hexing Road, Harbin 150040, China; E-Mail:;Asian Natural Environmental Science Center, University of Tokyo, Nishitokyo-shi, Tokyo 188-0002, Japan; E-Mail:

关键词: salt stress;    halophyte;    Puccinellia tenuiflora;    salt-tolerant FOX-yeast lines;   

DOI  :  10.3390/ijms150916469

来源: mdpi

【 摘 要 】

Puccinellia tenuiflora is a monocotyledonous halophyte that is able to survive in extreme saline soil environments at an alkaline pH range of 9–10. In this study, we transformed full-length cDNAs of P. tenuiflora into Saccharomyces cerevisiae by using the full-length cDNA over-expressing gene-hunting system to identify novel salt-tolerance genes. In all, 32 yeast clones overexpressing P. tenuiflora cDNA were obtained by screening under NaCl stress conditions; of these, 31 clones showed stronger tolerance to NaCl and were amplified using polymerase chain reaction (PCR) and sequenced. Four novel genes encoding proteins with unknown function were identified; these genes had no homology with genes from higher plants. Of the four isolated genes, two that encoded proteins with two transmembrane domains showed the strongest resistance to 1.3 M NaCl. RT-PCR and northern blot analysis of P. tenuiflora cultured cells confirmed the endogenous NaCl-induced expression of the two proteins. Both of the proteins conferred better tolerance in yeasts to high salt, alkaline and osmotic conditions, some heavy metals and H₂O₂ stress. Thus, we inferred that the two novel proteins might alleviate oxidative and other stresses in P. tenuiflora.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.