Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii

Marine Drugs

Liping Cai¹  Yanyan Li¹  Guanjun Tao¹  Wen Guo¹  Chan Zhang¹ 

[1] State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China; E-Mails:

关键词: Cronobacter sakazakii;    lipid A;    acyltransferase;    lpxL;    lpxM;    lpxP;   

DOI  :  10.3390/md11020377

来源: mdpi

【 摘 要 】

Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.