Comparative in Vivo Assessment of Some Adverse Bioeffects of Equidimensional Gold and Silver Nanoparticles and the Attenuation of Nanosilver’s Effects with a Complex of Innocuous Bioprotectors

International Journal of Molecular Sciences

Boris A. Katsnelson³  Larisa I. Privalova³  Vladimir B. Gurvich³  Oleg H. Makeyev⁴  Vladimir Ya. Shur¹  Yakov B. Beikin²  Marina P. Sutunkova³  Ekaterina P. Kireyeva³  Ilzira A. Minigalieva³  Nadezhda V. Loginova³  Marina S. Vasilyeva⁴  Artem V. Korotkov⁴  Eugene A. Shuman⁴  Larisa A. Vlasova⁴  Ekaterina V. Shishkina¹  Anastasia E. Tyurnina¹  Roman V. Kozin¹  Irene E. Valamina⁴  Svetlana V. Pichugova² 

[1] The Institute of Natural Sciences, the Ural Federal University, Ekaterinburg 620000, Russia; E-Mails:;The City Clinical Diagnostics Centre, 38 Dekabristov Str., Ekaterinburg 620142, Russia; E-Mails:;The Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, 30 Popov Str., Ekaterinburg 620014, Russia; E-Mails:;The Ural State Medical Academy, 17 Klyuchevskaya Str., Ekaterinburg 620109, Russia; E-Mails:

关键词: nanosilver;    nanogold;    bronchoalveolar lavage;    subchronic toxicity;    genotoxicity;    bioprotectors;   

DOI  :  10.3390/ijms14022449

来源: mdpi

【 摘 要 】

Stable suspensions of nanogold (NG) and nanosilver (NS) with mean particle diameter 50 and 49 nm, respectively, were prepared by laser ablation of metals in water. To assess rat’s pulmonary phagocytosis response to a single intratracheal instillation of these suspensions, we used optical, transmission electron, and semi-contact atomic force microscopy. NG and NS were also repeatedly injected intraperitoneally into rats at a dose of 10 mg/kg (0.5 mg per mL of deionized water) three times a week, up to 20 injections. A group of rats was thus injected with NS after oral administration of a “bioprotective complex” (BPC) comprised of pectin, multivitamins, some amino acids, calcium, selenium, and omega-3 PUFA. After the termination of the injections, many functional and biochemical indices and histopathological features of the spleen, kidneys and liver were evaluated for signs of toxicity, and accumulation of NG or NS in these organs was measured. From the same rats, we obtained cell suspensions of different tissues for performing the RAPD test. It was demonstrated that, although both nanometals were adversely bioactive in all respects considered in this study, NS was more noxious as compared with NG, and that the BPC tested by us attenuated both the toxicity and genotoxicity of NS.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.