Application of an Electrochemical Immunosensor with a MWCNT/PDAA Modified Electrode for Detection of Serum Trypsin

Sensors

Qiang Yi¹  Qicai Liu¹  Feng Gao³  Qingquan Chen² 

[1] Department of Laboratory Medicine, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China; E-Mail:;Department of Laboratory Medicine, College of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350005, China; E-Mails:;Department of Pathology, the 1st Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China; E-Mail:

关键词: electrochemical immunosensor;    trypsin;    multiwalled carbon nanotubes;    nanogold;   

DOI  :  10.3390/s140610203

来源: mdpi

【 摘 要 】

Objective: To establish an electrochemical immunosensor for the determination of serum trypsin levels using a multiwall carbon nanotubes (MWCNTs)-composite-modified electrode. Method: A MWCNT composite coated on the surface of bare gold electrodes was used for fixation of an anti-trypsin antibody. The assembly process and the performance indicators, including sensitivity, linear range of detection, anti-jamming performance, and stability, of the electrochemical immunosensor were examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Results: With optimized experimental conditions, the difference of the current value measured by differential pulse voltammetry (DPV) showed a linear relationship with the concentration of serum trypsin within 0.10–100 ng/mL. The detection limit for trypsin using this sensor was 0.002 ng/mL. Conclusions: The electrochemical immunosensor built using the MWCNT-composite-modified electrode is simple to operate and has a fast response time, along with a wide linear range, high sensitivity, and accuracy, making it suitable for serum trypsin detection.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.