A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

Sensors

Francesca Frascella⁴  Serena Ricciardi⁴  Paola Rivolo⁴  Valeria Moi⁴  Fabrizio Giorgis⁴  Emiliano Descrovi⁴  Francesco Michelotti¹  Peter Munzert³  Norbert Danz³  Lucia Napione²  Maria Alvaro² 

[1] Dipartimento di Scienze di Base e Applicate per l'Ingegneria, SAPIENZA Università di Roma, via A. Scarpa 16, Roma 00161, Italy; E-Mail:;Dipartimento di Scienze Oncologiche, Istituto per la Ricerca e la Cura del Cancro, Università di Torino, Candiolo 10060, Italy; E-Mails:;Fraunhofer Institute for Applied Optics and Precision Engineering, Albert-Einstein-Str. 7, Jena 07745, Germany; E-Mails:;Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, corso Duca degli Abruzzi 24, Torino 10129, Italy; E-Mails:

关键词: surface electromagnetic waves;    label-free sensing;    fluorescence;    multilayers;    bragg mirrors;   

DOI  :  10.3390/s130202011

来源: mdpi

【 摘 要 】

A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.