Preparation of an Immunoaffinity Column with Amino-Silica Gel Microparticles and Its Application in Sample Cleanup for Aflatoxin Detection in Agri-Products

Molecules

Fei Ma¹  Ran Chen¹  Peiwu Li¹  Qi Zhang¹  Wen Zhang¹ 

[1] ¹Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China

关键词: aflatoxins;    immunoaffinity column;    amino-silica gel;    microparticle conjugate;    agri-products;    HPLC;   

DOI  :  10.3390/molecules18022222

来源: mdpi

【 摘 要 】

This study established an immunoaffinity column for selective extraction of aflatoxins in agri-products. Specifically, the immunoaffinity column was developed by covalently coupling monoclonal antibody 1C11 against aflatoxins to amino-silica gel microparticles and then packing these into a cartridge. The extraction conditions were thoroughly optimized in terms of loading, washing and eluting solutions. Under the optimal conditions, the immunoaffinity column had a capacity of 200 ng of aflatoxins. The detection limits (S/N = 3) for aflatoxin G₁, B₁, G₂ and B₂ were 0.03, 0.07, 0.05 and0.09 μg·kg⁻¹, and the corresponding quantification limits (S/N = 10) were 0.10, 0.25, 0.18 and 0.30 μg·kg⁻¹, respectively. The recoveries of aflatoxins in samples were 90.1%–104.4% and RSDs were <4.4%. The developed method was further applied to the determination of aflatoxins in peanut, vegetable oil and tea samples, and the results indicated that peanut (26.9%), vegetable oils (28.0%) and tea (5.3%) samples were contaminated with aflatoxins, with levels ranging from 0.49 to 20.79 μg·kg⁻¹.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.