Ochratoxin A in brazilian instant coffee

Brazilian Journal of Microbiology

Adriana P. De Almeida¹  Janete Alaburda¹  Luzia Shundo¹  Valter Ruvieri¹  Sandra A. Navas¹  Leda C. A. Lamardo¹  Myrna Sabino¹ 

[1] ,Instituto Adolfo Lutz Seção de Química Biológica São Paulo SP ,Brasil

关键词: ochratoxin A;    instant coffee;    immunoaffinity column;    HPLC determination;    ocratoxina A;    café solúvel;    coluna de imunoafinidade;    determinação por cromatografia líquida de alta eficiência;   

DOI  :  10.1590/S1517-83822007000200022

来源: SciELO

【 摘 要 】

The aim of this study was to determine the ochratoxin A (OTA) contamination of instant coffee samples collected in the market of the city of São Paulo, Brazil from August to December, 2004. The EN 14133/2003 method, originally developed to quantify OTA in wine, grape juice and beer samples, was evaluated and approved for analyzing OTA in instant coffee samples. OTA was isolated in an immunoaffinity column and quantified by HPLC with fluorescence detection. The established detection and quantification limits were 0.16 and 0.52 ng/g, respectively. The recoveries from spiked samples were 92.6 ± 1.7, 83.7 ± 0.8, and 91.0 ± 1.2 % at levels of 3.0, 5.0, and 8.0 ng/g, respectively. Of a total of 82 samples analised, 81 (98.8%) contained OTA at levels ranging from 0.17 to 6.29 ng/g. The high frequency of OTA occurrence in the instant coffee samples demonstrates the importance of an effective control of this product by governmental authorities and industries. The rapid methodology for OTA analysis in instant coffee used in this study was defined and validated, permitting it´s use for quality control of this product.

【 授权许可】

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