Brazilin Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells

Molecules

Dae-Young Lee²  Mi-Kyoung Lee¹  Geum-Soog Kim²  Hyung-Jun Noh² 

[1] Department of Neurosurgery, Ajou University School of Medicine, Suwon, 443-721, Korea;Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA, Eumseong 369-873, Korea

关键词: apoptosis;    brazilin;    Caesalpinia sappan;    glioma U87;   

DOI  :  10.3390/molecules18022449

来源: mdpi

【 摘 要 】

Brazilin, isolated from the methanol extract of the heart wood of Caesalpinia sappan, sensitizes cancer cells to apoptosis. Glioblastoma multiforme (GBM), which accounts for most cases of central nervous system malignancy, has a very poor prognosis and lacks effective therapeutic interventions. We, therefore, investigated the effects of different concentrations of and different periods of exposure to brazilin on cell proliferation and apoptosis in the glioma U87 cell line. Cell proliferation was investigated by MTT assays and growth curve analysis, apoptosis was assessed by FACS analysis and western blot studies. Brazilin showed dose-dependent inhibition of cell proliferation and induction of apoptosis in glioma cells. It also increased the ratio of cleaved poly-(ADP)-ribose polymerase and decreased the expression of caspase-3 and caspase-7.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.