PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells

International Journal of Molecular Sciences

Jin-Young Kim⁴  Ramamourthy Gopal⁴  Sang Young Kim¹  Chang Ho Seo¹  Hyang Burm Lee²  Hyeonsook Cheong³ 

[1] Department of Bioinformatics, Kongju National University, Kongju 314-701, Korea; E-Mails:;College of Agriculture and Life Sciences, Chonnam National University, Gwangju 501-759, Korea; E-Mail:;Department of Biotechnology, Chosun University, Gwangju 501-759, Korea; E-Mail:;Research Center for Proteineous Materials, Chosun University, Gwangju 501-759, Korea; E-Mails:

关键词: peptide-G2;    RP-HPLC;    N-terminal sequence;    human pathogenic;    antimicrobial agent;   

DOI  :  10.3390/ijms14024349

来源: mdpi

【 摘 要 】

In an earlier study, we isolated potamin-1 (PT-1), a 5.6-kDa trypsin-chymotrypsin protease inhibitor, from the tubers of a potato strain (Solanum tuberosum L cv. Gogu Valley). We established that PT-1 strongly inhibits pathogenic microbial strains, but not human bacterial strains, and that its sequence shows 62% homology with a serine protease inhibitor. In the present study, we isolated an antifungal and antibacterial peptide with no cytotoxicity from tubers of the same potato strain. The peptide (peptide-G2, PG-2) was isolated using salt-extraction, ultrafiltration and reverse-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) showed the protein to have a molecular mass of 3228.5 Da, while automated Edman degradation showed the N-terminal sequence of PG-2 to be LVKDNPLDISPKQVQALCTDLVIRCMCCC-. PG-2 exhibited antimicrobial activity against Candida albicans, a human pathogenic yeast strain, and Clavibacter michiganensis subsp. michiganensis, a plant late blight strain. PG-2 also showed antibacterial activity against Staphylococcus aureus, but did not lyse human red blood cells and was thermostable. Overall, these results suggest PG-2 may be a good candidate to serve as a natural antimicrobial agent, agricultural pesticide and/or food additive.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.