Phytochemical profiles, antioxidant and antimicrobial activities of three Potentilla species

BMC Complementary and Alternative Medicine

Deng-Wu Li¹  Wen-Jun Pu¹  Dong-Mei Wang¹  Shan-Shan Wang¹ 

[1] College of Forestry, Northwest A & F University, Yangling, Shannxi 712100, China

关键词: RP-HPLC;    Antimicrobial activity;    Antioxidant activity;    Phytochemicals;    Potentilla spp;   

Others  :  1220519
DOI  :  10.1186/1472-6882-13-321

 received in 2013-09-05, accepted in 2013-11-15,  发布年份 2013

【 摘 要 】

Background

Extracts from Potentilla species have been applied in traditional medicine and exhibit antioxidant, hypoglycemic, anti-inflammatory, antitumor and anti-ulcerogenic properties, but little has been known about the diversity of phytochemistry and pharmacology on this genus. This study investigated and compared the phytochemical profiles, antioxidant and antimicrobial activities of leaf extracts from three Potentilla species (Potentilla fruticosa, Potentilla glabra and Potentilla parvifolia) in order to discover new resources for lead structures and pharmaceutical products.

Methods

Chemical composition and content of six phenolic compounds were evaluated and determined by RP-HPLC; Total phenolic and total flavonoid content were determined using Folin-Ciocalteau colourimetric method and sodium borohydride/chloranil-based method (SBC); Antioxidant activities were determined using DPPH, ABTS and FRAP assays; Antimicrobial properties were investigated by agar dilution and mycelial growth rate method.

Results

The results showed hyperoside was the predominant phenolic compound in three Potentilla species by RP-HPLC assay, with the content of 8.86 (P. fruticosa), 2.56 (P. glabra) and 2.68 mg/g (P. parvifolia), respectively. The highest content of total identified phenolic compounds (hyperoside, (+)-catechin, caffeic acid, ferulic acid, rutin and ellagic acid) was observed in P. parvifolia (14.17 mg/g), follow by P. fruticosa (10.01 mg/g) and P. glabra (7.01 mg/g). P. fruticosa possessed the highest content of total phenolic (84.93 ± 0.50 mmol gallic acid equivalent/100 g) and total flavonoid (84.14 ± 0.03 mmol quercetin equivalent/100 g), which were in good correlation with its significant DPPH_IC50 (16.87 μg/mL), ABTS (2763.48 μmol Trolox equivalent/g) and FRAP (1398.70 μmol Trolox equivalent/g) capacities. Furthermore, the effective methodology to distinguish the different species of Potentilla was also established by chromatographic fingerprint analysis for the first time. The results of antimicrobial activities showed P. fruticosa exhibited the strongest inhibition aganist Gram-positive bacteria, Pseudomonas aeruginosa and Candida albicans with MIC values of 0.78–6.25 mg/mL. P. parvifolia possessed antibacterial and antifungal activities against all the microorganisms tested, with EC₅₀ and MIC values of 20.52–47.02 mg/mL and 0.78–50 mg/mL, respectively.

Conclusions

These results indicated that leaf extracts from three Potentilla species could become useful supplement for pharmaceutical products as a new antioxidant and antimicrobial agents.

【 授权许可】

   
2013 wang et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

【 参考文献 】

• [1]Eriksson T, Donoghue MJ, Hibbs MS: Phylogenetic analysis of Potentilla using DNA sequences of nuclear ribosomal internal transcribed spacers (ITS), and implications for the classification of Rosoideae (Rosaceae). Plant Syst Evol 1998, 211:155-179.
• [2]Tomczyk M, Paduch R, Wiater A, Pleszczyńska M, Kandefer-szersze M, Szczodrak J: The influence of aqueous extracts of selected potentilla species on normal human colon cells. Acta Pol Pharm - Drug Research 2013, 70:523-531.
• [3]Gürbüz I, Özkan AM, Yesilada E, Kutsal O: Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi (Kayseri, Turkey). J Ethnopharmacol 2005, 101:313-318.
• [4]Leporatti ML, Ivancheva S: Preliminary comparative analysis of medicinal plants used in the traditional medicine of Bulgaria and Italy. J Ethnopharmacol 2003, 87:123-142.
• [5]Miliauskas G, van Beek TA, Venskutonis PR, Linssen JP, de Waard P, Sudhölter EJ: Antioxidant activity of Potentilla fruticosa. J Sci Food Agric 2004, 84:1997-2009.
• [6]Syiem D, Syngai G, Khup P, Khongwir B, Kharbuli B, Kayang H: Hypoglycemic effects of Potentilla fulgens L. in normal and alloxan-induced diabetic mice. J Ethnopharmacol 2002, 83:55-61.
• [7]Shushunov S, Balashov L, Kravtsova A, Krasnogorsky I, Latté KP, Vasiliev A: Determination of acute toxicity of the aqueous extract of Potentilla erecta (Tormentil) rhizomes in rats and mice. J Med Food 2009, 12:1173-1176.
• [8]Tomczyk M, Pleszczyńska M, Wiater A: Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity. Molecules 2010, 15:4639-4651.
• [9]Zhao Y-L, Cai G-M, Hong X, Shan L-M, Xiao X-H: Anti-hepatitis B virus activities of triterpenoid saponin compound from Potentilla anserine L. Phytomedicine 2008, 15:253-258.
• [10]Tomczyk M, Latté KP: Potentilla—A review of its phytochemical and pharmacological profile. J Ethnopharmacol 2009, 122:184-204.
• [11]Tomczyk M: Secondary metabolites from Potentilla recta L. and Drymocallis rupestris (L.) Soják (syn. Potentilla rupestris L.)(Rosaceae). Biochem Syst Ecol 2011, 39:893-896.
• [12]Barkley T, Cronquist A: A Synonymized Checklist of the Vascular Flora of the United States, Canada and Greenland. Volume II, The Biota of North America. Brittonia 1980, 32(4):573-573.
• [13]Shimono A, Ueno S, Gu S, Zhao X, Tsumura Y, Tang Y: Range shifts of Potentilla fruticosa on the Qinghai-Tibetan Plateau during glacial and interglacial periods revealed by chloroplast DNA sequence variation. Heredity 2009, 104:534-542.
• [14]Mitich L: Cinquefoils (Potentilla spp.): the five finger weeds. Weed Technology 1995, 9:857-861.
• [15]Miliauskas G, Venskutonis P, Van Beek T: Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004, 85:231-237.
• [16]Nkiliza J: Process for extracting catechin polyphenols from potentillas, extract obtained and its use. In Book Process for extracting catechin polyphenols from potentillas, extract obtained and its use. 1999. (Editor ed. eds.). City: US Patent 5,928,646
• [17]Wang LY, Ikeda H, Liu TL, Wang YJ, Liu JQ: Repeated Range Expansion and Glacial Endurance of Potentilla glabra (Rosaceae) in the Qinghai‒Tibetan Plateau. J Integr Plant Biol 2009, 51:698-706.
• [18]Wang L-Y, Kou Y-X, Wu G-L, Wang Y-J: Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species—Potentilla glabra L. Conserv Genet Resour 2009, 1:51-53.
• [19]Davidson CG, Lenz LM: Experimental taxonomy of Potentilla fruticosa. Can J Bot 1989, 67:3520-3528.
• [20]Yang J, Martinson TE, Liu RH: Phytochemical profiles and antioxidant activities of wine grapes. Food Chem 2009, 116:332-339.
• [21]Duncan CE, Gorbet DW, Talcott ST: Phytochemical content and antioxidant capacity of water-soluble isolates from peanuts (Arachis hypogaea L.). Food Res Int 2006, 39:898-904.
• [22]Singleton V, Rossi JA: Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticul 1965, 16:144-158.
• [23]He X, Liu D, Liu RH: Sodium borohydride/chloranil-based assay for quantifying total flavonoids. J Agric Food Chem 2008, 56:9337-9344.
• [24]Yen G-C, Chen H-Y: Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 1995, 43:27-32.
• [25]Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999, 26:1231-1237.
• [26]Benzie IF, Strain J: The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 1996, 239:70-76.
• [27]Bansod S, Rai M: Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigatus and A. niger. World J Med Sci 2008, 3(2):81-88.
• [28]Hsu F-L, Chen P-S, Chang H-T, Chang S-T: Effects of alkyl chain length of gallates on their antifungal property and potency as an environmentally benign preservative against wood-decay fungi. Int Biodeter Biodegr 2009, 63(5):543-547.
• [29]Tian J, Ban X, Zeng H, He J, Huang B, Wang Y: Chemical composition and antifungal activity of essential oil from Cicuta virosa L. var. latisecta Celak. Int J Food Microbiol 2011, 145(2):464-470.
• [30]Falleh H, Ksouri R, Chaieb K, Karray-Bouraoui N, Trabelsi N, Boulaaba M, Abdelly C: Phenolic composition of Cynara cardunculus L. organs, and their biological activities. C R Biol 2008, 331:372-379.
• [31]Tomczyk M, Leszczyńska K, Jakoniuk P: Antimicrobial activity of Potentilla species. Fitoterapia 2008, 79(7):592-594.
• [32]Chandrasekaran M, Venkatesalu V: Antibacterial and antifungal activity of Syzygium jambolanum seeds. J Ethnopharmacol 2004, 91(1):105-108.
• [33]Martini S, D’Addario C, Colacevich A, Focardi S, Borghini F, Santucci A, Figura N, Rossi C: Antimicrobial activity against Helicobacter pylori strains and antioxidant properties of blackberry leaves (Rubus ulmifolius) and isolated compounds. Int J Antimicrob Agents 2009, 34(1):50-59.
• [34]Orhan I, Özçelik B, Kartal M, Özdeveci B, Duman H: HPLC quantification of vitexine-2′-O-rhamnoside and hyperoside in three Crataegus species and their antimicrobial and antiviral activities. Chromatographia 2007, 66(1):153-157.