Simultaneous Determination of Flavonoids, Isochlorogenic Acids and Triterpenoids in Ilex hainanensis Using High Performance Liquid Chromatography Coupled with Diode Array and Evaporative Light Scattering Detection

Molecules

Bo Peng²  Chun-Feng Qiao²  Jing Zhao²  Wei-Hua Huang²  De-Jun Hu²  Hua-Gang Liu¹ 

[1] School of Pharmaceutical Sciences, Guangxi Medical University, Nanning 530021, China;State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China

关键词: Ilex hainanensis;    flavonoid;    isochlorogenic acid;    triterpenoid;    HPLC-DAD-ELSD;   

DOI  :  10.3390/molecules18032934

来源: mdpi

【 摘 要 】

A high performance liquid chromatography coupled with diode array and evaporative light scattering detection (HPLC-DAD-ELSD) method for simultaneous determination of eight major bioactive compounds including two flavonoids (rutin and eriodictyol-7-O-β-d-glucopyranoside), two isochlorogenic acids (isochlorogenic acid A and isochlorogenic acid C) and four triterpenoids (ilexhainanoside D, ilexsaponin A₁, ilexgenin A and ursolic acid) in Ilex hainanensis has been developed for the first time. The 283 nm wavelength was chosen for determination of two flavonoids and two isochlorogenic acids. ELSD was applied to determine four triterpenoids. The analysis was performed on an Agilent Zorbax SB-C₁₈ column (250 × 4.6 mm i.d., 5 µm) with gradient elution of 0.2% formic acid in water and acetonitrile. The method was validated for linearity, limit of detection, limit of quantification, precision, repeatability and accuracy. The proposed method has been successfully applied for simultaneous quantification of the analytes in four samples of Ilex hainanensis, which is helpful for quality control of this plant.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.