Evaluation of Macroalgae Sulfated Polysaccharides on the Leishmania (L.) amazonensis Promastigote

Marine Drugs

Camila Lehnhardt Pires²  Selma Dzimidas Rodrigues²  Daniel Bristot²  Henrique Hessel Gaeta²  Daniela de Oliveira Toyama³  Wladimir Ronald Lobo Farias¹ 

[1] Pici Unit, Federal University of Ceará, UFC, Av. Mister Hull, s/n, Bloco 827, Fortaleza, CE, 60356-000, Brazil; E-Mail:;São Vicente Unit, State University of São Paulo Julio Mesquita Filho, UNESP, Praça Infante Dom Henrique, s/n, São Vicente 11330-900, SP, Brazil; E-Mails:;Center of Biological and Health Sciences, Mackenzie Presbyterian University, Rua da Consolação, 896, São Paulo 01302-907, SP, Brazil; E-Mail:

关键词: macroalgae;    Leishmania (L.) amazonensis;    sulfated polysaccharides;    Solieria filiformis;    Botryocladia occidentalis;    Caulerpa racemosa;    Gracilaria caudate;   

DOI  :  10.3390/md11030934

来源: mdpi

【 摘 要 】

The sulfated polysaccharides from Solieria filiformis (Sf), Botryocladia occidentalis (Bo), Caulerpa racemosa (Cr) and Gracilaria caudata (Gc) were extracted and extensively purified. These compounds were then subjected to in vitro assays to evaluate the inhibition of these polysaccharides on the growth of Leishmania (L.) amazonensis promastigotes. Under the same assay conditions, only three of the four sulfated polysaccharides were active against L. amazonensis, and the polysaccharide purified from Cr was the most potent (EC₅₀ value: 34.5 μg/mL). The polysaccharides derived from Bo and Sf demonstrated moderate anti-leishmanial activity (EC₅₀ values of 63.7 μg/mL and 137.4 μg/mL). In addition, we also performed in vitro cytotoxic assays toward peritoneal macrophages and J774 macrophages. For the in vitro cytotoxicity assay employing J774 cells, all of the sulfated polysaccharides decreased cell survival, with CC₅₀ values of 27.3 μg/mL, 49.3 μg/mL, 73.2 μg/mL, and 99.8 μg/mL for Bo, Cr, Gc, and Sf, respectively. However, none of the sulfated polysaccharides reduced the cell growth rate of the peritoneal macrophages. These results suggest that macroalgae contain compounds with various chemical properties that can control specific pathogens. According to our results, the assayed sulfated polysaccharides were able to modulate the growth rate and cell survival of Leishmania (L.) amazonensis promastigotes in in vitro assays, and these effects involved the interaction of the sulfated polysaccharides on the cell membrane of the parasites.

【 授权许可】

CC BY   
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