Antiproliferative Activity of Fucan Nanogel

Marine Drugs

Nednaldo Dantas-Santos¹  Jailma Almeida-Lima¹  Arthur Anthunes Jacome Vidal¹  Dayanne Lopes Gomes¹  Ruth Medeiros Oliveira¹  Silvia Santos Pedrosa²  Paula Pereira²  Francisco Miguel Gama² 

[1] Laboratory of Biotechnology of Natural Polymers (BIOPOL), Departament of Biochemistry, Federal University of Rio Grande do Norte (UFRN), Natal-RN 59078-970, Brazil;IBB—Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Minho University, Braga 4704-553, Portugal;

关键词: nanogels;    cancer cells;    sulfated polysaccharides;    Spatoglossum schröederi;   

DOI  :  10.3390/md10092002

来源: mdpi

【 摘 要 】

Sulfated fucans comprise families of polydisperse natural polysaccharides based on sulfated L-fucose. Our aim was to investigate whether fucan nanogel induces cell-specific responses. To that end, a non toxic fucan extracted from Spatoglossum schröederi was chemically modified by grafting hexadecylamine to the polymer hydrophilic backbone. The resulting modified material (SNFuc) formed nanosized particles. The degree of substitution with hydrophobic chains was close to 100%, as estimated by elemental analysis. SNFfuc in aqueous media had a mean diameter of 123 nm and zeta potential of −38.3 ± 0.74 mV, as measured by dynamic light scattering. Nanoparticles conserved their size for up to 70 days. SNFuc cytotoxicity was determined using the MTT assay after culturing different cell lines for 24 h. Tumor-cell (HepG2, 786, H-S5) proliferation was inhibited by 2.0%–43.7% at nanogel concentrations of 0.05–0.5 mg/mL and rabbit aorta endothelial cells (RAEC) non-tumor cell line proliferation displayed inhibition of 8.0%–22.0%. On the other hand, nanogel improved Chinese hamster ovary (CHO) and monocyte macrophage cell (RAW) non-tumor cell line proliferation in the same concentration range. The antiproliferative effect against tumor cells was also confirmed using the BrdU test. Flow cytometric analysis revealed that the fucan nanogel inhibited 786 cell proliferation through caspase and caspase-independent mechanisms. In addition, SNFuc blocks 786 cell passages in the S and G2-M phases of the cell cycle.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.