Lack of Genomic Instability in Bone Marrow Cells of SCID Mice Exposed Whole-Body to Low-Dose Radiation

International Journal of Environmental Research and Public Health

Kanokporn Noy Rithidech²  Chatchanok Udomtanakunchai¹  Louise Honikel² 

[1]Department of Radiologic Technology, Faculty of Associated Medical Sciences, Center of Excellence for Molecular Imaging, Chiang Mai University, Chiang Mai 50200, Thailand

[2] E-Mail:

[3]Pathology Department, Stony Brook University, Stony Brook, NY 11974, USA

[4] E-Mail:

关键词: low-dose radiation;    SCID mouse;    bone marrow cells;    genomic instability;    chromosome aberrations;   

DOI  :  10.3390/ijerph10041356

来源: mdpi

【 摘 要 】

It is clear that high-dose radiation is harmful. However, despite extensive research, assessment of potential health-risks associated with exposure to low-dose radiation (at doses below or equal to 0.1 Gy) is still challenging. Recently, we reported that 0.05 Gy of ¹³⁷Cs gamma rays (the existing limit for radiation-exposure in the workplace) was incapable of inducing significant in vivogenomic instability (measured by the presence of late-occurring chromosomal damage at 6 months post-irradiation) in bone marrow (BM) cells of two mouse strains, one with constitutively high and one with intermediate levels of the repair enzyme DNA-dependent protein-kinase catalytic-subunit (DNA-PKcs). In this study, we present evidence for a lack of genomic instability in BM cells of the severely combined-immunodeficiency (SCID/J) mouse (which has an extremely low-level of DNA-PKcs activity) exposed whole-body to low-dose radiation (0.05 Gy). Together with our previous report, the data indicate that low-dose radiation (0.05 Gy) is incapable of inducing genomic instability in vivo (regardless of the levels of DNA-PKcs activity of the exposed mice), yet higher doses of radiation (0.1 and 1 Gy) do induce genomic instability in mice with intermediate and extremely low-levels of DNA-PKcs activity (indicating an important role of DNA-PKcs in DNA repair).

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.