Micellar Electrokinetic Chromatography with Laser-Induced Fluorescence Detection for Separation of Red and Yellow Historical Dyes

Chromatography

Shokoufeh Ahmadi¹  Douglas B. Craig² 

[1] Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; E-Mail:;Department of Chemistry, Richardson College for the Environment and Science Complex, University of Winnipeg, 599 Portage Ave., Winnipeg, MB R3B 2G3, Canada; E-Mail:

关键词: micellar electrokinetic chromatography (MEKC);    laser-induced fluorescence (LIF);    anthraquinone;    flavonoid;    historical dyes;   

DOI  :  10.3390/chromatography1010009

来源: mdpi

【 摘 要 】

In this study, the separation parameters in micellar electrokinetic chromatography (MEKC)-laser-induced fluorescence (LIF) were optimized for the separation of red and yellow historical dyes in 20 mM borate buffer with 20 mM sodium dodecyl sulfate (SDS). Separation conditions were optimized by changing pH, organic modifier (methanol and acetonitrile) concentrations and applied voltage. The mixtures of dyes used in this study included four anthraquinone dyes (alizarin, purpurin, emodin and carmine) and six flavonoid dyes (luteolin, apigenin, kaempferol, quercetin, morin and myricetin). For this work, dyes were introduced electro-kinetically (10 kV for 5 s) into a 50 cm capillary (10 µm id) and separated using a running potential of 18, 20, 22 and 25 kV. Absolute limits of detection for most of these dyes was less than 1 pg. For dyes such as alizarin, improved detection limits were achieved at pH = 9.24; however dyes such as purpurin had significantly improved detection limits at pH = 8.0. The successful extraction and identification of a number of dyes in plants and textiles samples is also described.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.