An Optofluidic Temperature Probe

Sensors

Ilona Węgrzyn¹  Alar Ainla¹  Gavin David Michael Jeffries¹ 

[1] Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, Göteborg SE-412 96, Sweden;

关键词: Rhodamine B;    Rhodamine 6G;    multifunctional pipette;    microfluidic device;    microthermometer;    temperature sensing;    semi-contact;    optofluidic;    TRPV1;   

DOI  :  10.3390/s130404289

来源: mdpi

【 摘 要 】

We report the application of a microfluidic device for semi-contact temperature measurement in picoliter volumes of aqueous media. Our device, a freely positionable multifunctional pipette, operates by a hydrodynamic confinement principle, i.e., by creating a virtual flow cell of micrometer dimensions within a greater aqueous volume. We utilized two fluorescent rhodamines, which exhibit different fluorescent responses with temperature, and made ratiometric intensity measurements. The temperature dependence of the intensity ratio was calibrated and used in a model study of the thermal activation of TRPV1 ion channels expressed in Chinese hamster ovary cells. Our approach represents a practical and robust solution to the specific problem of measuring temperature in biological experiments in vitro, involving highly localized heat generation, for example with an IR-B laser.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.