Coupling between proteolytic processing and translocation of the precursor of the F1AT Pase β‐subunit during its import into mitochondria of intact cells

FEBS Letters

Hatalová, Irena¹  Kolarov, Jordan¹ 

[1] Cancer Research Institute, Slovak Academy of Sciences, Čs.armády 21, 812 32 Bratislava, Czechoslovakia

关键词: Precursor protein;    Mitochondrial protein transport;    Processing protease;    Rhodamine 6G;    F1-ATPase;    PMSF;    phenylmethylsulfonyl fluoride;    βF1;    β-subunit of mitochondrial F1-ATPase;    R6G;    rhodamine 6G;    pre-βF1;    precursor of β-subunit of mitochondrial F1-ATPase;   

DOI  :  10.1016/0014-5793(84)81262-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The intracellular transport of newly synthesized β-subunits of the F₁-ATPase (βF₁) and of newly synthesized ADP/ATP carrier was followed in isolated rat hepatoma cells. As tested by rapid fractionation of [³⁵S]methionine pulse- and pulse-chase-labeled cells and by sensitivity of labeled polypeptides to externally added protease, the import of βF₁ into mitochondria was strongly inhibited by the additional low concentrations of rhodamine 6G (R6G). In contrast, the import of the ADP/ATP carrier into mitochondria was not affected by the inhibitor. The results imply that the proteolytic processing of the precursor of βF₁, is coupled to its translocation across the mitochondrial membrane.

【 授权许可】

Unknown