Optimization of Hydrogen Peroxide Detection for a Methyl Mercaptan Biosensor

Sensors

Zhan-Hong Li¹  Houssemeddine Guedri²  Bruno Viguier²  Shi-Gang Sun¹ 

[1] State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China; E-Mails:;Laboratoire IMAGES EA 4218, Groupe Biocapteurs, Université de Perpignan Via Domitia (UPVD), Bâtiment S, 52 av Paul Alduy 66860 Perpignan Cedex, France; E-Mails:

关键词: methyl mercaptan;    hydrogen peroxide;    amperometric sensor;    screen printed electrode;   

DOI  :  10.3390/s130405028

来源: mdpi

【 摘 要 】

Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H₂O₂) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H₂O₂, applying a potential of +0.6 V, +0.6 V, +0.4 V, −0.2 V and −0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H₂O₂ were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H₂O₂ at a working potential as low as −0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.