A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

Sensors

Ana Lorena Alvarado-Gámez¹  Mar໚ Asunción Alonso-Lomillo²  Olga Domínguez-Renedo² 

[1] CELEQ and School of Chemistry, University of Costa Rica, San Pedro de Montes de Oca, San José P.O. Box 11500-2060, Costa Rica;Department of Chemistry, Faculty of Sciences, University of Burgos, Plaza Misael Bañuelos s/n, Burgos 09001, Spain; E-Mails:

关键词: alkaline phosphatase;    biosensor;    gold nanoparticles;    4-nitrophenyl phosphate;    screen printed electrode;    vanadium;    water analysis;   

DOI  :  10.3390/s140203756

来源: mdpi

【 摘 要 】

A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 μM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.