Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae

Marine Drugs

A. Catarina Guedes²  Maria S. Gião²  Rui Seabra¹  A. C. Silva Ferreira²  Paula Tamagnini¹  Pedro Moradas-Ferreira¹ 

[1] IBMC—Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre nº 823, Porto P-4150-180, Portugal; E-Mails:;CBQF/Biotechnology College, Catholic University of Portugal, Rua Dr. António Bernardino de Almeida, Porto P-4200-072, Portugal; E-Mails:

关键词: 16S rDNA;    ABTS^•+;    deoxyribose;    DNA;    phage P22/Salmonella;    Ames test;   

DOI  :  10.3390/md11041256

来源: mdpi

【 摘 要 】

A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H₂O₂. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.