Microbial Biosensors: Engineered Microorganisms as the Sensing Machinery

Sensors

Miso Park¹  Shen-Long Tsai² 

[1] Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA; E-Mail:;Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan; E-Mail:

关键词: synthetic biology;    scaffolds;    genetic circuits;   

DOI  :  10.3390/s130505777

来源: mdpi

【 摘 要 】

Whole-cell biosensors are a good alternative to enzyme-based biosensors since they offer the benefits of low cost and improved stability. In recent years, live cells have been employed as biosensors for a wide range of targets. In this review, we will focus on the use of microorganisms that are genetically modified with the desirable outputs in order to improve the biosensor performance. Different methodologies based on genetic/protein engineering and synthetic biology to construct microorganisms with the required signal outputs, sensitivity, and selectivity will be discussed.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.