Presence of Viral Genome in Urine and Development of Hematuria and Pathological Changes in Kidneys in Common Marmoset (Callithrix jacchus) after Inoculation with Dengue Virus

Pathogens

Meng Ling Moi⁴  Tsutomu Omatsu⁴  Takanori Hirayama⁴  Shinichiro Nakamura¹  Yuko Katakai²  Tomoyuki Yoshida³  Akatsuki Saito³  Shigeru Tajima⁴  Mikako Ito⁴  Tomohiko Takasaki⁴  Hirofumi Akari³ 

[1] Shiga University of Medical Science, Seta Tsukinowa-cho, Otsu, Shiga, 520-2192, Japan; E-Mail:;National Institute of Biomedical Innovation, 1 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan; E-Mail:;Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan; E-Mails:;National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan; E-Mails:

关键词: dengue virus;    animal model;    marmoset;    urine;    hematuria;    kidney;   

DOI  :  10.3390/pathogens2020357

来源: mdpi

【 摘 要 】

Common marmosets (Callithrix jacchus) developed high levels of viremia, clinical signs including fever, weight loss, a decrease in activity and hematuria upon inoculation with dengue virus (DENV). Presence of DENV genome in urine samples and pathological changes in kidneys were examined in the present study. Levels of DENV genome were determined in 228 urine samples from 20 primary DENV-inoculated marmosets and in 56 urine samples from four secondary DENV-inoculated marmosets. DENV genome was detected in 75% (15/20) of marmosets after primary DENV infection. No DENV genome was detected in urine samples from the marmosets with secondary infection with homologous DENV (0%, 0/4). Two marmosets demonstrated hematuria. Pathological analysis of the kidneys demonstrated non-suppressive interstitial nephritis with renal tubular regeneration. DENV antigen-positive cells were detected in kidneys. In human dengue virus infections, some patients present renal symptoms. The results indicate that marmosets recapitulate some aspects of the involvement of kidneys in human DENV infection, and suggest that marmosets are potentially useful for the studies of the pathogenesis of DENV infection, including kidneys.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.