The Marmoset as an Animal Model of Influenza: Infection With A(H1N1)pdm09 and Highly Pathogenic A(H5N1) Viruses via the Conventional or Tracheal Spray Route

Frontiers in Microbiology

Machiko Horiuchi¹  Kenta Takahashi²  Noriko Nakajima²  Hideki Hasegawa²  Yoshihiro Kawaoka³  Kiyoko Iwatsuki-Horimoto⁴  Maki Kiso⁴  Mutsumi Ito⁴  Erika Sasaki⁶  Norio Okahara⁷  Takashi Inoue⁷ 

[1] BioSciences Group, Summit Pharmaceuticals International Corporation, Tokyo, Japan;Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan;Department of Special Pathogens, International Research Center for Infectious Diseases, Institute of Medical Science, University of Tokyo, Tokyo, Japan;Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan;Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United States;Keio Advanced Research Center, Keio University, Tokyo, Japan;Marmoset Research Department, Central Institute for Experimental Animals, Kawasaki, Japan;

关键词: influenza virus;    animal model;    marmoset;    non-human primate;    A(H5N1);   

DOI  :  10.3389/fmicb.2018.00844

来源: DOAJ

【 摘 要 】

To control infectious diseases in humans, it is important to understand the pathogenicity of the infecting organism(s). Although non-human primates, such as cynomolgus and rhesus macaques, have been used for influenza virus infection models, their size can limit their use in confined animal facilities. In this study, we investigated the susceptibility of marmosets to influenza viruses to assess the possibility of using these animals as a non-human primate model for influenza research. We first used an influenza A (H1N1)pdm09 virus to compare two inoculation routes: the conventional route, via a combination of the intratracheal, intranasal, ocular, and oral routes; and the tracheal spray route. In marmosets inoculated via the tracheal spray route, we found inflammation throughout the lungs and trachea. In contrast, in marmosets inoculated via the conventional route, the inflammation was confined to roughly the center of the lung. These data suggest that the tracheal spray route may be more suitable than the conventional route to inoculate marmosets with influenza viruses. We also tested an influenza A(H5N1) highly pathogenic avian influenza (HPAI) virus and found that some marmosets inoculated with this virus via the tracheal spray route showed weight loss, decreased body temperature, and loss of appetite and activity. The replication of this H5N1 virus in respiratory organs was confirmed. These results indicate the potential of marmosets as an animal model for infection with seasonal or HPAI viruses.

【 授权许可】

Unknown