Non-Covalent Synthesis of Metal Oxide Nanoparticle–Heparin Hybrid Systems: A New Approach to Bioactive Nanoparticles

International Journal of Molecular Sciences

Elena Vismara³  Antonio Valerio³  Alessia Coletti³  Giangiacomo Torri¹  Sabrina Bertini¹  Giorgio Eisele¹  Rosalba Gornati² 

[1] Ronzoni Institute for Chemical and Biochemical Research, 81 G. Colombo Street, 20133 Milan, Italy; E-Mails:;Interuniversity Center “The Protein Factory,” Polytechnic of Milan, ICRM-CNR Milan and Insubria University, 21100 Varese, Italy;Department of Chemistry, Materials and Chemical Engineering “G. Natta” Polytechnic, 7 Mancinelli Street, 20131 Milan, Italy; E-Mails:

关键词: heparin;    transition metal oxide nanoparticles;    organic inorganic nanostructures;    electrostatic interaction;    NMR spectroscopy;    microscopy;   

DOI  :  10.3390/ijms140713463

来源: mdpi

【 摘 要 】

Heparin has been conjugated to Fe₃O₄, Co₃O₄, and NiO nanoparticles (NPs) through electrostatic interactions, producing colloidal suspensions of hybrid metal oxide heparin NPs that are stable in water. Negative zeta potentials and retention of heparin’s ability to capture toluidine blue indicate that heparin’s negative charges are exposed on the surface of the coated NPs. IR results confirmed the formation of nanohybrids as did NMR experiments, which were also interpreted on the basis of toluidine blue tests. Transmission electron microscopy results revealed that the heparin coating does not modify the shape or dimension of the NPs. Dynamic light scattering and negative zeta potential measurements confirmed that heparin surface functionalisation is an effective strategy to prevent NP aggregation.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland