Microfluidic Platform for Enzyme-Linked and Magnetic Particle-Based Immunoassay

Micromachines

Nikhil Bhalla¹  Danny Wen Yaw Chung¹  Yaw-Jen Chang³  Kimberly Jane S. Uy¹  Yi Ying Ye¹  Ting-Yu Chin²  Hao Chun Yang¹ 

[1] Department of Electronic Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan; E-Mails:;Department of Biotechnology, Chung Yuan Christian University, Zhongli 32023, Taiwan; E-Mail:;Department of Mechanical Engineering, Chung Yuan Christian University, Zhongli 32023, Taiwan; E-Mail:

关键词: microfluidics;    magnetic bead;    immunoassay;    Hall Effect;    Bio-MEMS;    bead–protein complex;   

DOI  :  10.3390/mi4020257

来源: mdpi

【 摘 要 】

This article presents design and testing of a microfluidic platform for immunoassay. The method is based on sandwiched ELISA, whereby the primary antibody is immobilized on nitrocelluose and, subsequently, magnetic beads are used as a label to detect the analyte. The chip takes approximately 2 h and 15 min to complete the assay. A Hall Effect sensor using 0.35-μm BioMEMS TSMC technology (Taiwan Semiconductor Manufacturing Company Bio-Micro-Electro-Mechanical Systems) was fabricated to sense the magnetic field from the beads. Furthermore, florescence detection and absorbance measurements from the chip demonstrate successful immunoassay on the chip. In addition, investigation also covers the Hall Effect simulations, mechanical modeling of the bead–protein complex, testing of the microfluidic platform with magnetic beads averaging 10 nm, and measurements with an inductor-based system.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.