Enhanced Development of Azoxymethane-Induced Colonic Preneoplastic Lesions in Hypertensive Rats

International Journal of Molecular Sciences

Takahiro Kochi²  Masahito Shimizu²  Tomohiko Ohno²  Atsushi Baba²  Takafumi Sumi²  Masaya Kubota²  Yohei Shirakami²  Hisashi Tsurumi²  Takuji Tanaka¹ 

[1] Department of Tumor Pathology, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan; E-Mail:;Department of Internal Medicine, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan; E-Mails:

关键词: hypertension;    colon carcinogenesis;    oxidative stress;    inflammation;    angiotensin-II;   

DOI  :  10.3390/ijms140714700

来源: mdpi

【 摘 要 】

Metabolic syndrome is associated with an increased risk of colorectal cancer. This study investigated the impact of hypertension, a component of metabolic syndrome, on azoxymethane (AOM)-induced colorectal carcinogenesis using SHRSP/Izm (SHRSP) non-diabetic/hypertensive rats and SHRSP.Z-Lepr^fa/IzmDmcr (SHRSP-ZF) diabetic/hypertensive rats. Male 6-week-old SHRSP, SHRSP-ZF, and control non-diabetic/normotensive Wister Kyoto/Izm (WKY) rats were given 2 weekly intraperitoneal injections of AOM (20 mg/kg body weight). Two weeks after the last injection of AOM, the SHRSP and SHRSP-ZF rats became hypertensive compared to the control WKY rats. Serum levels of angiotensin-II, the active product of the renin-angiotensin system, were elevated in both SHRSP and SHRSP-ZF rats, but only the SHRSP-ZF rats developed insulin resistance, dyslipidemia, and hyperleptinemia and exhibited an increase in adipose tissue. The development of AOM-induced colonic preneoplastic lesions and aberrant crypts foci, was significantly accelerated in both SHRSP and SHRSP-ZF hypertensive rats, compared to WKY normotensive rats. Furthermore, induction of oxidative stress and exacerbation of inflammation were observed in the colonic mucosa and systemically in SHRSP and SHRSP-ZF rats. Our findings suggest that hypertension plays a role in the early stage of colorectal carcinogenesis by inducing oxidative stress and chronic inflammation, which might be associated with activation of the renin-angiotensin system.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland