HPLC Analysis of Phenolics Compounds and Antioxidant Capacity of Leaves of Vitex megapotamica (Sprengel) Moldenke

Molecules

Thiele Faccim de Brum⁴  Marina Zadra⁴  Mariana Piana⁴  Aline Augusti Boligon⁴  Janaina Kieling Fröhlich⁴  Robson Borba de Freitas⁴  Sílvio Terra Stefanello¹  Amanda Luana Forbrig Froeder⁴  Bianca Vargas Belke²  Letໜia Teixeira Nunes²  Roberta da Silva Jesus⁴  Michel Mansur Machado³  João Batista Teixeira da Rocha¹  Félix Alexandre Antunes Soares¹ 

[1] Post-Graduate Program in Biochemical Toxicology, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS, 97105-900, Brazil;Pharmacy Course, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS 97105-900, Brazil;Post-Graduate Program in Pharmaceutical Sciences, Federal University of Pampa-UNIPAMPA, Uruguaiana, RS 97500-970, Brazil;Post-Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS, 97105-900, Brazil

关键词: Vitex megapotamica;    phenolic compounds;    antioxidant capacity;    HPLC/DAD;   

DOI  :  10.3390/molecules18078342

来源: mdpi

【 摘 要 】

Vitex megapotamica (Sprengel) Moldenke belongs to the Verbenaceae family and is popularly known as “tarumã”. The antioxidant capacity of fractions and crude extract from the leaves of V. megapotamica were determined in this study through the capacity to remove reactive species and phenolic compounds were quantified in the various fractions. The IC₅₀ (DPPH) ranged from 14.17 ± 0.76 to 37.63 ± 0.98 µg/mL. The ethyl acetate fraction might contain the strongest lipid peroxidation inhibitory compounds with an IC₅₀ of 16.36 ± 5.09 µg/mL, being also the one with the highest content of polyphenols (522.4 ± 1.12 mg/g), flavonoids (220.48 ± 0.30 mg/g) and condensed tannins (3.86 ± 0.53 mg/g). Compounds quantified by HPLC/DAD in the crude extract and fractions were chlorogenic and rosmarinic acids. Higher dosages of the extracts were more effective in reducing levels of plasma protein carbonyls and were also shown to be able to remove reactive species by a 2',7'-dichlorofluorescein diacetate assay, reducing oxidative stress in all tested fractions. Results obtained indicated that V. megapotamica exhibits good potential to prevent diseases caused by the overproduction of free radicals and it might also be used as a potential source of natural antioxidant agents.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.