Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling

Molecules

Lei Wang²  Wataru Hisano²  Yuta Murai²  Munenori Sakurai²  Yasuyuki Muto²  Haruka Ikemoto²  Masashi Okamoto²  Takashi Murotani²  Reika Isoda²  Dongyeop Kim²  Yasuko Sakihama²  Irnayuli R. Sitepu³  Yasuyuki Hashidoko²  Yasumaru Hatanaka¹ 

[1] Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan;Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University; Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan;Forest Microbiology Laboratory, Forest and Nature Conservation Research and Development Center, Forest Research and Development Agency, Jalan Gunung Batu No.5, P.O. Box 165, Bogor 16610, Indonesia

关键词: photoaffinity label;    phenylalanine;    tryptophan;    phenyl azide;    benzophenone;    trifluoromethyldiazirine;    metabolites;   

DOI  :  10.3390/molecules18078393

来源: mdpi

【 摘 要 】

Photoaffinity labeling is a reliable analytical method for biological functional analysis. Three major photophores—aryl azide, benzophenone and trifluoromethyldiazirine—are utilized in analysis. Photophore-bearing l-phenylalanine derivatives, which are used for biological functional analysis, were inoculated into a Klebsiella sp. isolated from the rhizosphere of a wild dipterocarp sapling in Central Kalimantan, Indonesia, under nitrogen-limiting conditions. The proportions of metabolites were quite distinct for each photophore. These results indicated that photophores affected substrate recognition in rhizobacterial metabolic pathways, and differential photoaffinity labeling could be achieved using different photophore-containing l-phenylalanine derivatives.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.