Cytotoxic Effects of Hydroxylated Fullerenes in Three Types of Liver Cells

Materials

Kumiko Shimizu¹  Reiji Kubota¹  Norihiro Kobayashi¹  Maiko Tahara¹  Naoki Sugimoto²  Tetsuji Nishimura¹ 

[1] Division of Environmental Chemistry, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan; E-Mails:;Division of Food Additives, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo 158-8501, Japan; E-Mail:

关键词: hydroxylated fullerene;    C₆₀;    cytotoxic activity;    liver cells;    mitochondrial damage;   

DOI  :  10.3390/ma6072713

来源: mdpi

【 摘 要 】

Fullerenes C₆₀ have attracted considerable attention in the biomedical field due to their interesting properties. Although there has been a concern that C₆₀ could be metabolized to hydroxylated fullerenes (C₆₀(OH)_x) in vivo, there is little information on the effect of hydroxylated C₆₀ on liver cells. In the present study, we evaluated the cytotoxic effects of fullerene C₆₀ and various hydroxylated C₆₀ derivatives, C₆₀(OH)₂, C₆₀(OH)_6–12, C₆₀(OH)₁₂ and C₆₀(OH)₃₆, with three different types of liver cells, dRLh-84, HepG2 and primary cultured rat hepatocytes. C₆₀, C₆₀(OH)₂ and C₆₀(OH)₃₆ exhibited little or no cytotoxicity in all of the cell types, while C₆₀(OH)_6–12 and C₆₀(OH)₁₂ induced cytotoxic effects in dRLh-84 cells, accompanied by the appearance of numerous vacuoles around the nucleus. Moreover, mitochondrial activity in liver cells was significantly inhibited by C₆₀(OH)_6–12 and C₆₀(OH)₁₂. These results indicate that the number of hydroxyl groups on C₆₀(OH)_x contribute to the difference of their cytotoxic potential and mitochondrial damage in liver cells.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.