Inhibitory Effect of Heparin on Collagen Fiber Formation in Hepatic Cells in Culture

Cell Structure and Function

Kenji Sorimachi³  Masashi Ikeda²  Kazumi Akimoto¹ 

[1]Research Center for Molecular and Cellular Biology, Dokkyo University School of Medicine

[2]Laboratory of Medical Sciences, Dokkyo University School of Medicine

[3]Department of Microbiology, Dokkyo University School of Medicine

关键词: collagen;    hydroxyproline;    glycosaminoglycan;    heparin;    liver cells;   

DOI  :  10.1247/csf.22.533

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(24)Cited-By(1)When M cells derived from rat liver and then transformed by treatment with 4-dimethylamino-azobenzene were cultured in vitro, the culture became covered with a collagen fiber network. When the M cells were cultured in the presence of more than 10 μg/ml heparin, no collagen fiber formation was observed. The inhibitory effect was evident at 5 μg/ml but was not significant at 1 μg/ml. High performance liquid chromatography (HPLC) showed that about 50% decrease in hydroxyproline content occurred in the presence of 10 μg/ml heparin. The inhibition by heparin reached a plateau at 10 μg/ml. Other glycosaminoglycans such as heparan sulfate, keratan sulfate, chondroitin sulfate B and chondroitin sulfate C did not show a significant effect on hydroxyproline content. Modified heparins slightly decreased hydroxyproline content, but the collagen fibers were still observed. These results indicate that the native structure of heparin is important to attain the complete inhibition of collagen fiber formation; the basic structure, (-GlcUA or IdUA β 1-4 GlcNSO3-)n, is important. HPLC, Northern blot analysis and Western blot analysis for rat type I collagen revealed that collagen synthesis is independent of heparin, but that collagen fiber formation is prevented by heparin.

【 授权许可】

Unknown